|
|
|
| Registros recuperados: 46 | |
|
| |
|
| |
|
|
| Zhao,Shan; Jiang,Qian-Tao; Ma,Jian; Wang,Ji-Rui; Liu,Ya-Xi; Chen,Guo-Yue; Qi,Peng-Fei; Pu,Zhi-En; Lu,Zhen-Xiang; Zheng,You-Liang; Wei,Yu-Ming. |
| The WUSCHEL (WUS)-related homeobox (WOX) gene family coordinates transcription during the early phases of embryogenesis. In this study, a putative WOX2 homolog was isolated and characterized from Aegilops tauschii, the donor of D genome of Triticum aestivum. The sequence consisted of 2045 bp, and contained an open reading frame (ORF), encoded 322 amino acids. The predicted protein sequence contained a highly conserved homeodomain and the WUS-box domain, which is present in some members of the WOX protein family. The full-length ORF was subcloned into prokaryotic expression vector pET-30a, and an approximately 34-kDa protein was expressed in Escherichia coli BL21 (DE3) cells with IPTG induction. The molecular mass of the expressed protein was identical to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: WOX2; Aegilops tauschii; Phylogenetic analysis; Real-time quantitative PCR. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100079 |
| |
|
|
| Krapp-Schickel, T.; Koenemann, S.. |
| The amphipod family Stenothoidae contains more than 200 species in about 40 genera; these genera are at present often defined not by the presence, but by the absence of synapomorphies, thus defining grades rather than clades. Our phylogenetic analyses yielded 4 groups of stenothoids: a basic proboloidid clade; an advanced and always clearly separated Austral-Antarctic thaumatelsonid clade, with a possibly related Arctic mesometopid clade; and finally a poorly resolved group, the stenothoids sensu stricto, including the large and probably polyphyletic genera Stenothoe and Metopa, each with more than 50 species. It is proposed to study and analyse these groups separately in future, based on better redescriptions of the individual species. Our analyses... |
| Tipo: Article / Letter to the editor |
Palavras-chave: Taxonomy; Amphipoda; Stenothoidae; Thaumatelsonidae; Phylogenetic analysis; 42.74. |
| Ano: 2006 |
URL: http://www.repository.naturalis.nl/record/214545 |
| |
|
|
| Li,Huixin; Liu,Shengwang; Han,Zongxi; Shao,Yuhao; Chen,Shuhong; Kong,Xiangang. |
| The nucleotide sequences of eight open reading frames (ORFs) located at the 5' end of the unique long region of the duck enteritis virus (DEV) Clone-03 strain were determined. The genes identified were designated UL1 , UL2 , UL3 , UL4 , UL5 , UL6 and UL 7 homologues of the herpes simplex virus 1 (HSV-1). The DEV UL3.5 located between UL3 and UL4 had no homologue in the HSV-1. The arrangement and transcription orientation of the eight genes were collinear with their homologues in the HSV-1. Phylogenetic trees were constructed based on the alignments of the deduced amino acids of eight proteins with their homologues in 12 alpha-herpesviruses. In the UL1, UL3, UL3.5, UL5 and UL7 proteins trees, the branches were more closely related to the genus... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Duck enteritis virus; UL1-UL7 genes; Phylogenetic analysis. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100018 |
| |
|
| |
|
|
| Jaklitsch, W.M.; Samuels, G.J.; Ismaiel, A.; Voglmayr, H.. |
| Trichoderma viridescens is recognised as a species complex. Multigene analyses based on the translation elongation factor 1-alpha encoding gene (tef1), a part of the rpb2 gene, encoding the second largest RNA polymerase subunit and the larger subunit of ATP citrate lyase (acl1) reveals 13 phylogenetic species with little or no phenotypic differentiation. This is the first use of acl1 in Trichoderma phylogenetics. The typification of T. viridescens s.str. is clarified and Hypocrea viridescens is replaced by the new name T. paraviridescens. Besides these two species, eleven are phylogenetically recognised and T. olivascens, T. viridarium, T. virilente, T. trixiae, T. viridialbum, T. appalachiense, T. neosinense, T. composticola, T. nothescens and T.... |
| Tipo: Article / Letter to the editor |
Palavras-chave: Acl1; Hypocrea; Hypocreaceae; Phylogenetic analysis; Rpb2; Systematics; Tef1. |
| Ano: 2013 |
URL: http://www.repository.naturalis.nl/record/532266 |
| |
|
|
| Cunha,L.K.H.; Kashima,S.; Amarante,M.F.C.; Haddad,R.; Rodrigues,E.S.; Silva,K.L.T.; Lima,T.A.; Castro,D.B.; Brito,F.C.; Almeida,E.G.; Covas,D.T.; Malheiro,A.. |
| Few studies have reported the molecular epidemiological characterization of HIV-1 in the Northern region of Brazil. The present study reports the molecular and epidemiological characterization of 31 HIV-1 isolates from blood donors from the State of Amazonas who donated blood between April 2006 and March 2007. Serum/plasma samples from all donors were screened for HIV antibodies by ELISA and the results confirmed by Western blot analysis. Genomic DNA was extracted from the buffy coat using the Super Quik-Gene-DNA Isolation kit. Nested PCR was performed on the env, gag, and pol regions of HIV-1 using the Gene Amp PCR System 9700. Sequencing reactions were performed using the inner PCR primers and the DYEnamic™ ET Dye Terminator Kit, and phylogenetic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HIV-1; Subtypes; Phylogenetic analysis; Blood donors; Molecular and epidemiological characterization. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200003 |
| |
|
|
| Rabello,Aline R.; Queiroz,Paulo R.; Simões,Kenya C.C.; Hiragi,Cássia O.; Lima,Luzia H.C.; Oliveira,Maria Regina V.; Mehta,Angela. |
| The Bemisia tabaci complex is formed by approximately 41 biotypes, two of which (B and BR) occur in Brazil. In this work we aimed at obtaining genetic markers to assess the genetic diversity of the different biotypes. In order to do that we analyzed Bemisia tabaci biotypes B, BR, Q and Cassava using molecular techniques including RAPD, PCR-RFLP and sequencing of the ITS1 rDNA region. The analyses revealed a high similarity between the individuals of the B and Q biotypes, which could be distinguished from the BR individuals. A phylogenetic tree based on ITS1 rDNA sequence was constructed. This is the first report of the ITS1 rDNA sequence of Bemisia tuberculata and of the BR biotype of B. tabaci. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Whitefly; ITS rDNA; Genetic diversity; Phylogenetic analysis. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300029 |
| |
|
|
| García,Adriana; Rhoden,Sandro A; Rubin Filho,Celso J; Nakamura,Celso V; Pamphile,João A. |
| Endophytic fungi inhabit vegetable tissues or organs, without causing them any harm. Endophytes can co-evolve with plant hosts and possess species-specific interactions. They can protect the plant from insect attacks and diseases, and are also able to produce substances of biotechnological interest. In folk medicine, the bark, roots and fruits of Sapindus saponaria is used to produce substances with anxiolytic, astringent, diuretic and expectorant properties, as well as tonics, blood depuratives and cough medicine. This study evaluated the diversity of endophytic fungi present in the leaves of S. saponaria L. and observed the colonization of host plants by endophytes, using light and scanning electron microscopy. We verified that these fungi are found in... |
| Tipo: Journal article |
Palavras-chave: Endophyte; Light microscopy; Phylogenetic analysis; SEM. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200006 |
| |
|
|
| Kothe, Caroline; Delbarre-ladrat, Christine; Renault, Pierre; Passerini, Delphine. |
| Halophilic and psychrophilic marine bacteria are source of interesting bioactive molecules for biotechnology. We report here the whole-genome sequences of two of them, Pseudoalteromonas sp. MIP2626 isolated from tropical peeled shrimps and Psychrobacter sp. BI730 isolated from deep-sea hydrothermal vent. Sequencing of both genomes was performed by Illumina HiSeq platform (2 × 150 pb). De novo assemblies using Spades v3.9 generated 136 contigs for Pseudoalteromonas MIP2626 and 42 contigs for Psychrobacter BI730, representing a genome size of 3.9 Mb and 3.2 Mb, respectively. Phylogenetic based on 16S rRNA gene sequence and phylogenomic analyses were reported to compare the new sequences with Pseudoalteromonas and Psychrobacter representative strains... |
| Tipo: Text |
Palavras-chave: Aquatic; Genomic; Phylogenetic analysis; Halophilic. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00635/74662/74568.pdf |
| |
|
|
| Aşkar,S. |
| ABSTRACT Chicken infectious anemia (CIA) is an immune-suppressive disease caused by chicken anemia virus (CAV). It is characterized by lymphoid atrophy, aplastic anemia, especially in chicks. In this study, full-length genomic characterization of CAV DNA from the broiler flocks in Turkey and phylogenetic analysis were aimed. In the study, CAV DNA were found positive for 37 (53%) flocks with PCR studies from thymus tissues of each 70 broiler flocks. And 17 purified CAV DNA PCR products from these 37 CAV isolates were full length sequenced with the NGS method (Illumina MiSeq). Also with the phylogenetic analyses, full length PCR products of 17 purified CAV isolates have been determined as 2298bp genome size and 99% similarity with each other. The highest... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Broiler; Chicken Infectious Anemia; Phylogenetic analysis. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000300323 |
| |
|
|
| Médici,K.C.; Barry,A.F.; Alfieri,A.F.; Alfieri,A.A.. |
| Group B rotaviruses (RV-B) were first identified in piglet feces, being later associated with diarrhea in humans, cattle, lambs, and rats. In human beings, the virus was only described in China, India, and Bangladesh, especially infecting adults. Only a few studies concerning molecular analysis of the RV-B NSP2 gene have been conducted, and porcine RV-B has not been characterized. In the present study, three porcine wild-type RV-B strains from piglet stool samples collected from Brazilian pig herds were used for analysis. PAGE results were inconclusive for those samples, but specific amplicons of the RV-B NSP2 gene (segment 8) were obtained in a semi-nested PCR assay. The three porcine RV-B strains showed the highest nucleotide identity with the human WH1... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Piglets; Diarrhea; Group B rotavirus; NSP2 gene; Phylogenetic analysis. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100003 |
| |
|
| |
|
|
| Ishida,Juliane Karine; Caldas,Danielle G.G.; Oliveira,Lucas Roberto; Frederici,Gabriela Campos; Leite,Lucas Margato Pereira; Mui,Tsai Siu. |
| Abstract Transporter proteins play an essential role in the uptake, trafficking and storage of metals in plant tissues. The Natural Resistance-Associated Macrophage Protein (NRAMP) family plays an essential role in divalent metal transport. We conducted bioinformatics approaches to identify seven NRAMP genes in the Phaseolus vulgaris genome, investigated their phylogenetic relation, and performed transmembrane domain and gene/protein structure analyses. We found that the NRAMP gene family forms two distinct groups. One group included the PvNRAMP1, -6, and -7 genes that share a fragmented structure with a numerous exon/intron organization and encode proteins with mitochondrial or plastidial localization. The other group is characterized by few exons that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phaseolus vulgaris; Transporter; NRAMP; Gene family; Phylogenetic analysis. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500820 |
| |
|
|
| Hu,Lifang; Liu,Shiqiang. |
| Members of the ERF transcription-factor family participate in a number of biological processes, viz., responses to hormones, adaptation to biotic and abiotic stress, metabolism regulation, beneficial symbiotic interactions, cell differentiation and developmental processes. So far, no tissue-expression profile of any cucumber ERF protein has been reported in detail. Recent completion of the cucumber full-genome sequence has come to facilitate, not only genome-wide analysis of ERF family members in cucumbers themselves, but also a comparative analysis with those in Arabidopsis and rice. In this study, 103 hypothetical ERF family genes in the cucumber genome were identified, phylogenetic analysis indicating their classification into 10 groups, designated I to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cucumis sativus L.; ERF; Phylogenetic analysis; Transcription factor; Genome sequence. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400015 |
| |
|
|
| Kumar,Pradeep; Kamle,Madhu; Misra,Asok Kumar; O'Donovan,Anthonia; Pagano,Marcela; Modi,Dinesh Raj. |
| ABSTRACT Fusarium mangiferae (=F. subglutinans) isolates collect from malformed samples from major mango-growing area of North India. Molecular identification and characterization of eleven most virulent isolates of F. mangiferae, based on pathogenicity tests used for the present study. Species-specific, genus specific ITS-PCR and PCR-RFLP performed for the accurate and easy detection of F. mangiferae. The rDNA-ITS 28S region sequences used for phylogenetic analysis of Fusarium isolates from India and other countries for homology search between them. The phylogenetic tree divided the isolates into three clades (i.e., American, Asian and African) and showed the high level of sequence based similarity (69-99%) among all Fusarium sequences from Asia. Thus,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fusarium mangiferae; Detection; Phylogenetic analysis; Vegetative malformation; Floral malformation.. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100204 |
| |
|
|
| Rocha,Graça Celeste Gomes; Corrêa,Régis Lopes; Borges,Anna Cristina Neves; Sá,Claudio Bustamante Pereira de; Alves-Ferreira,Márcio. |
| Homeobox genes encode transcriptional factors, usually involved in molecular control of plant developmental patterns. They can be divided into several classes according to conserved sequences within the homeobox region and the presence of specific additional sequences. Based on these conserved sequences, we developed a search procedure to identify possible homeobox genes in the Eucalyptus Genome Sequencing Project Consortium (FORESTs) database. We were able to identify 50 Eucalyptus sequences (EST-contigs) containing the homeodomain sequence. Phylogenetic analysis was applied to this ESTs-contigs and 44 of them were found to have similarities with one of three well-known homeobox classes: Bell, Knox and HD-Zip, and their sub-classes. However, no EST-contig... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Plant development; Abiotic stress; Eucalyptus; FORESTs; Phylogenetic analysis. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400005 |
| |
|
|
| Inglis,Peter W.; Tigano,Myrian S.. |
| A phylogenetic analysis of the 5.8S rDNA and internal transcribed spacer (ITS1 and ITS2) sequences from some entomogenous Paecilomyces species supports the polyphyly of the genus and showed the existence of cryptic species. In the Eurotiales, anamorphs Paecilomyces variotii and Paecilomyces leycettanus were related to the teleomorphs Talaromyces and Thermoascus. In the Hypocreales, three major ITS subgroups were found, one of which included Paecilomyces viridis, Paecilomyces penicillatus, Paecilomyces carneus and isolates identified as Paecilomyces lilacinus and Paecilomyces marquandii. However, the majority of the P. lilacinus and P. marquandii isolates formed a distinct and distantly related subgroup, while the other major subgroup contained Paecilomyces... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Paecilomyces spp.; Phylogenetic analysis; RDNA; Molecular taxonomy. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100025 |
| |
|
|
| Martins,Maira de S.N.; Castro,Alessandra M.M.G. de; Lima,Michele dos S.; Pinto,Vivian da S.C.; Silva,Thaís G. da; Fava,Claudia Del; Depes,Claudio Regis; Okuda,Liria H.; Pituco,Edviges M.. |
| Abstract Malignant Catarrhal Fever (MCF) was investigated in the central nervous system of cattle with neurological syndrome. Two-hundred-ninety samples were analyzed by histology, and molecular methods to detect ovine herpesvirus type 2 (OvHV-2) were optimized and validated. The qualitative polymerase chain reaction (qualitative PCR) analytical sensitivity was 101 DNA copies/µL and found 4.8% (14/290) positive for OvHV-2. The quantitative polymerase chain reaction (qPCR) analytical sensitivity was 100 DNA copy/µL and 5.9% (17/290) positivity, with 47.1% (8/17) of the positive samples presenting histological evidence of non-purulent meningo-encephalitis. The qualitative PCR products (422 bp of the ORF75 region) were sequenced and submitted to phylogenetic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ovine herpesvirus type 2 (OvHV-2); Histopathology; Qualitative PCR; Quantitative PCR; Phylogenetic analysis. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200366 |
| |
| Registros recuperados: 46 | |
|
|
|
